As of Wednesday, travellers and tourists no longer have to present a negative test for coronavirus or proof of illness or vaccination when entering Italy.
The earlier restriction expired on Tuesday and will not be extended, the Italian Ministry of Health stated.
The government of Prime Minister Mario Draghi has been phasing out epidemiological measures since a two-year state of emergency ended in late March.
Covid certificates are no longer needed in restaurants or tourist events.
People still have to wear masks in public transport, hospitals and care facilities.
The number of confirmed coronavirus cases has been declining in recent weeks in Italy, a country of nearly 60 million people. Authorities reported just over 7,500 new cases within 24 hours and approximately 60 deaths on Monday.